Related Experiment Video
Updated: Oct 26, 2025

Roughness Impact of Piezoelectric Dental Scaler on Two Distinct Flowable Composite Filling Materials
Published on: January 10, 2025
Influence of Bleaching Gel Peroxide Concentration on Color and Penetration through the Tooth Structure
Carlos Rg Torres1, Rayssa F Zanatta2, Monique Mm Godoy3
1Department of Restorative Dentistry, Institute of Science and Technology, Sao Paulo State University-UNESP, São José dos Campos, São Paulo, Brazil, Phone: +55 12 3947 9376,
Aim And Objective:
The purpose of this study was to assess the effect of hydrogen peroxide concentration on the bleaching efficacy and penetration through the tooth structure.
Materials And Methods:
One hundred enamel/dentin specimens with cylindrical shape were obtained from bovine incisors. The surfaces were polished and the size standardized. They were divided into five groups (n = 20), following the concentration of hydrogen peroxide in the bleaching gels: 20, 25, 30, 35, and 40% (w/w). The specimens were placed over artificial pulpal chambers containing acetate buffer solution and bleached for 30 minutes (three applications of 10 minutes each). Aliquots of the acetate solution were collected, and the peroxide concentration was measured by an analytic spectrophotometer. The color of the samples was analyzed using a colorimetric spectrophotometer at the baseline and 7 days after the bleaching procedure. The color difference was calculated using the ∆Eab formula. The data were analyzed by one-way ANOVA and Tukey's test (p <0.05).
Results:
The peroxide concentrations of 20-30% showed smaller bleaching effect than the higher concentrations (p = 0.001). The peroxide penetration was significantly higher (p = 0.001) for the more concentrated gels (35 and 40%).
Conclusion:
The higher peroxide concentrations enhance the bleaching efficacy, but also increased the peroxide penetration through the tooth structure.
Clinical Significance:
In-office bleaching gels with higher concentrations of hydrogen peroxide (35 and 40%) present superior whitening efficacy. Nevertheless, they might also intensify the negative biological effects on the pulpal tissue, since they exhibit increased penetration potential. How to cite this article: Torres CRG, Zanatta RF, Godoy MMM, et al. Influence of Bleaching Gel Peroxide Concentration on Color and Penetration through the Tooth Structure. J Contemp Dent Pract 2021;22(5):479-483.
More Related Videos
14:52Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
Published on: November 24, 2012
07:04Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Related Concept Videos
Changes in Skin Color: Clinical Perspectives
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...