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Updated: Oct 26, 2025

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Capillary-Mediated Single-Cell Dispenser.

Li Wu1,2, Shihan Xu1, Jingang Wang1

  • 1Department of Chemistry and Bioengineering, University of Washington, Seattle, Washington 98195, United States.

Analytical Chemistry
|July 28, 2021
PubMed
Summary
This summary is machine-generated.

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We developed an easy-to-assemble single-cell dispenser for multiomics studies. This device accurately sorts and dispenses single cells into multiwell plates for downstream analysis like PCR.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity in various biological processes.
  • Current methods for single-cell isolation and dispensing can be complex and time-consuming.
  • Advancements in microfluidic technologies are needed to improve efficiency and accuracy in single-cell workflows.

Purpose of the Study:

  • To develop and optimize a novel single-cell dispenser for accurate sorting and dispensing of individual cells.
  • To adapt electrohydrodynamic jet printing principles for precise droplet generation and cell encapsulation.
  • To demonstrate the utility of the developed dispenser in preparing samples for single-cell molecular analyses.

Main Methods:

  • A single-cell dispenser was designed using fused silica capillary tubing as both an optical detection window and dispensing nozzle.

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  • Electrohydrodynamic jet printing principles were employed for droplet generation and cell manipulation.
  • Systematic optimization of parameters including capillary dimensions, flow rate, applied voltage, and solution properties was performed using COMSOL simulations and experimental validation.
  • The dispenser was assembled and tested for its ability to sort and dispense single cells into multiwell plates.

    • Main Results:

    • The developed single-cell dispenser achieved accurate droplet generation with volumes of 5-10 nL.
    • Optimization using 100-μm inner diameter and 160-μm outer diameter capillary tubing resulted in efficient single-cell encapsulation and dispensing.
    • The system demonstrated successful sorting and dispensing of single cells into multiwell plates.
    • The dispenser proved to be easy to assemble and operate.

    Conclusions:

    • The novel single-cell dispenser, inspired by electrohydrodynamic jet printing, offers a precise and efficient solution for single-cell manipulation.
    • The use of fused silica capillary tubing simplifies the device design and enhances functionality.
    • This technology facilitates downstream single-cell analyses, such as PCR, by enabling accurate cell dispensing into multiwell formats.
    • The developed dispenser represents a valuable tool for advancing single-cell multiomics research.