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In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Published on: November 20, 2018

Hepta-Histidine Inhibits Tau Aggregation.

Kanoh Kondo¹, Teikichi Ikura², Hikari Tanaka¹

¹Department of Neuropathology, Medical Research Institute and Center for Brain Integration Research, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

ACS Chemical Neuroscience

|July 28, 2021

Summary

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Hepta-histidine (7H) inhibits Tau aggregation in vitro and reduces Tau phosphorylation in neurons. This peptide shows promise as a therapeutic for Tau-related neurodegenerative diseases like Alzheimer's disease.

Area of Science:

Neuroscience
Molecular Biology
Drug Discovery

Background:

Tau aggregation is a key feature of neurodegenerative diseases, including Alzheimer's disease (AD).
Targeting Tau aggregation is a significant strategy for therapeutic development in tauopathies.

Purpose of the Study:

To investigate the potential of hepta-histidine (7H) as an inhibitor of Tau aggregation.
To evaluate the efficacy of a cell-permeable TAT-7H conjugate in neuronal models of tauopathies.

Main Methods:

In vitro assays to assess the effect of 7H on Tau-R3 peptide aggregation.
Synthesis of a TAT-7H conjugate to enhance cellular uptake.
Treatment of induced pluripotent stem cell (iPSC)-derived neurons with TAT-7H to evaluate Tau phosphorylation.

Keywords:

Hepta-histidine TAT aggregation neurodegenerative diseases tau

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Last Updated: Oct 26, 2025

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Published on: November 20, 2018

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Published on: February 27, 2018

Main Results:

Hepta-histidine (7H) demonstrated suppression of Tau-R3 peptide aggregation in vitro.
The TAT-7H conjugate exhibited increased cell permeability.
TAT-7H treatment reduced Tau phosphorylation in iPSC-derived neurons with Tau or APP mutations.

Conclusions:

Hepta-histidine (7H) is a potential lead compound for developing anti-aggregation drugs.
TAT-7H represents a promising therapeutic strategy for Tau-related neurodegenerative diseases, including Alzheimer's disease.