Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

African hamster lymphocytes as a model for immunologic studies.

H M Howell, J L Hall

    Research in Veterinary Science
    |November 1, 1977
    PubMed
    Summary

    The white-tailed rat (Mystromys albicaudatus) shows a unique splenic lymphocyte mitogenic response, incorporating [3H] thymidine at low cell densities. This distinct immune profile suggests its potential as a novel model for immunologic research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sugar unloading in roots of Ricinus communis L.: III. The extravascular pathway of sugar transport.

    The New phytologist·2021
    Same author

    Sugar unloading in roots of Ricinus communis L.: II. Characteristics of the extravascular apoplast.

    The New phytologist·2021
    Same author

    Sugar unloading in roots of Ricinus communis L.: I. The characteristics of enzymes concerned with sucrose catabolism and a comparison of, their distribution in root and shoot tissues.

    The New phytologist·2021
    Same author

    Solute transport into healthy and powdery mildew-infected leaves of pea and uptake by powdery mildew mycelium.

    The New phytologist·2021
    Same author

    Transversus abdominis muscle as a gastric or intestinal on-lay flap in two dogs and one cat.

    The Journal of small animal practice·2021
    Same author

    A novel internal abdominal oblique muscle flap to close a major abdominal wall defect.

    The Journal of small animal practice·2019

    Area of Science:

    • Immunology
    • Comparative immunology
    • Animal models for research

    Background:

    • The white-tailed rat (Mystromys albicaudatus) is a rodent species.
    • Understanding immune responses in different animal models is crucial for advancing immunologic research.

    Purpose of the Study:

    • To investigate the mitogenic response of splenic lymphocytes from Mystromys albicaudatus.
    • To evaluate the potential of Mystromys albicaudatus as a model for immunologic research.

    Main Methods:

    • Splenic lymphocytes were isolated from Mystromys albicaudatus.
    • Cells were stimulated with mitogens such as phytohemagglutinin (PHA) and concanavalin A (ConA).
    • Lymphocyte proliferation was measured by [3H] thymidine incorporation.

    Main Results:

    • Mystromys splenic cells showed optimal [3H] thymidine incorporation at 10^5 to 2 x 10^5 cells/microculture in response to PHA and ConA.
    • Maximum incorporation levels exceeded 10^5 cpm, even at low cell concentrations.
    • Little to no incorporation was observed with lipopolysaccharide (LPS) or purified protein derivative (PPD) at doses effective in mice.

    Conclusions:

    • The unique mitogenic response profile of Mystromys lymphocytes, particularly high proliferation at low cell densities, distinguishes it from common mouse strains.
    • These characteristics suggest potential applications for studying mechanisms of lymphocyte activation and mitogenesis.
    • Mystromys albicaudatus may serve as a valuable, distinct model for specific immunologic investigations.

    Related Experiment Videos