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The introduction of polyesters has brought major development to the textile industry. The wrinkle-free behavior of polyester blends has eliminated the need for starching and ironing clothes.
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Tailor-Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila.

Mengyi Li1,2, Yueyuan Ma1, Xu Zhang1

  • 1School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Advanced Materials (Deerfield Beach, Fla.)
|July 29, 2021
PubMed
Summary
This summary is machine-generated.

Researchers engineered Pseudomonas entomophila to produce novel polyhydroxyalkanoate (PHA) copolymers. This breakthrough enables the synthesis of custom short- and medium/long-chain-length PHA (SCL-co-MCL/LCL PHA) with improved properties.

Keywords:
Pseudomonas entomophilaSCL-co-LCL PHA copolymerSCL-co-MCL PHA copolymerflux tuningmetabolic engineeringpolyhydroxyalkanoatessynthetic biology

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Area of Science:

  • Microbiology
  • Biotechnology
  • Polymer Science

Background:

  • Microbial polyhydroxyalkanoates (PHAs) are biopolyesters with tunable properties.
  • Synthesizing copolymers with both short-chain-length (SCL) and medium/long-chain-length (MCL/LCL) monomers (SCL-co-MCL/LCL PHAs) is challenging due to enzyme specificity and precursor limitations.
  • PHAs with monomers longer than nine carbons are particularly difficult to produce.

Purpose of the Study:

  • To develop a microbial platform for synthesizing novel SCL-co-MCL/LCL PHA copolymers.
  • To overcome limitations in producing PHAs with longer carbon chain monomers.
  • To engineer a Pseudomonas entomophila strain for efficient PHA production.

Main Methods:

  • Reprogramming a β-oxidation weakened Pseudomonas entomophila strain.
  • Introducing synthesis pathways for SCL 3-hydroxybutyryl-CoA (3HB) from glucose.
  • Introducing synthesis pathways for MCL/LCL 3-hydroxyalkanoyl-CoA from fatty acids (C9-C18).
  • Utilizing a low specificity PHA synthase (PhaC61-3) for polymerization.
  • Applying rational flux-tuning strategies.

Main Results:

  • The engineered P. entomophila successfully synthesized P(3HB-co-MCL/LCL 3HA) copolymers.
  • Optimized strains accumulated 55 wt% poly-3-hydroxybutyrate (PHB) at 8.4 g L-1 cell dry weight.
  • Novel SCL-co-MCL/LCL PHA copolymers were synthesized with over 60 wt% PHA at 9 g L-1 cell dry weight.
  • The modified P. entomophila serves as a high-performing platform for tailor-made PHA production.

Conclusions:

  • The study presents a successful reprogramming of P. entomophila for novel PHA copolymer synthesis.
  • Weakened β-oxidation and tailored precursor supply enable efficient production of SCL-co-MCL/LCL PHAs.
  • This engineered platform opens avenues for generating custom biopolyesters with desirable properties.