Draft Genome Sequence of a bla_KPC-2-Carrying Citrobacter braakii Isolate from Pediatric Hospital Wastewater in Peru

Area of Science:

• Microbiology
• Genomics
• Environmental Science

Background:

• The emergence of multidrug-resistant (MDR) bacteria poses a significant threat to public health.
• Carbapenemase-producing Enterobacteriaceae (CPE), such as those carrying the blaKPC gene, are of particular concern due to limited treatment options.
• Hospital effluent can serve as a potential pathway for the dissemination of antibiotic resistance genes (ARGs) into the environment.

Purpose of the Study:

• To report the draft genome sequence of a blaKPC-2-carrying Citrobacter braakii isolate.
• To characterize a multidrug-resistant C. braakii strain from a clinical environment in Peru.

Main Methods:

• Whole-genome sequencing of the Citrobacter braakii isolate (CF248).
• Bioinformatic analysis to identify relevant genes, including blaKPC-2.
• Isolation from pediatric hospital effluent.

Main Results:

• A draft genome sequence of a blaKPC-2-carrying Citrobacter braakii isolate, designated CF248, was obtained.
• The isolate was identified as multidrug-resistant.
• The isolate was recovered from pediatric hospital effluent in Peru.

Conclusions:

• The genome sequence provides valuable data for understanding the genetic basis of multidrug resistance in C. braakii.
• This study underscores the importance of monitoring hospital wastewater for the presence of ARGs.
• Environmental surveillance is crucial for tracking the spread of resistant bacteria in clinical settings.