Related Experiment Video
Updated: Oct 26, 2025
08:10
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Published on: June 24, 2019
27.0K
Complete Genome Sequence of Pseudomonas Phage Zikora
Chiemeke Grace Ezemokwe1, Francis Maji Agwom2, Ijeoma Okoliegbe3
1African Centre of Excellence in Phytomedicine Research and Development, University of Jos, Jos, Plateau State, Nigeria.
Microbiology Resource Announcements
|July 29, 2021
Summary
Researchers isolated the Pseudomonas bacteriophage Zikora, a novel virus targeting the common pathogen Pseudomonas aeruginosa. This discovery offers new insights into phage diversity and potential antimicrobial strategies against resistant bacteria.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Pseudomonas aeruginosa is a significant pathogen in humans and animals.
- The bacterium often exhibits resistance to conventional antimicrobial drugs.
Purpose of the Study:
- To isolate and characterize a novel bacteriophage targeting Pseudomonas aeruginosa.
- To determine the genomic features and taxonomic classification of the isolated phage.
Main Methods:
- Isolation of bacteriophage from environmental samples.
- Whole-genome sequencing of the isolated bacteriophage.
- Bioinformatic analysis for genome annotation and phylogenetic comparison.
Main Results:
- Successful isolation of Pseudomonas bacteriophage Zikora.
- The complete genome of Zikora (65,837 bp) was sequenced and annotated.
- Zikora shares genomic similarities with Pbunavirus phages, indicating it is a new member of this genus within the Myoviridae family.
Conclusions:
- Pseudomonas bacteriophage Zikora represents a novel addition to the Pbunavirus genus.
- This finding expands our understanding of Pseudomonas phage diversity.
- The characterization of Zikora may contribute to developing alternative therapeutic approaches against Pseudomonas aeruginosa infections.
Related Concept Videos
01:26DNA Bacteriophages
313
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
313
00:43Lysogenic Cycle of Bacteriophages
64.5K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
64.5K
01:30Lytic Cycle of Bacteriophages
73.5K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
73.5K
01:16Viral Replication: Lysogenic Cycle
509
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
509
00:46Genomic DNA in Prokaryotes
45.7K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
45.7K
01:20Viral Replication: Lytic Cycle
557
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
557