Related Experiment Video
Updated: Oct 26, 2025

12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
5.4K
Complete Genome Sequence of Pantoea agglomerans ASB05 Using Illumina and PacBio Sequencing
Sang In Lee1, Thao D Tran2, Steven Huynh3
1Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, USA.
Microbiology Resource Announcements
|July 29, 2021
Abstract:
We present the complete genome sequence of Pantoea agglomerans ASB05 and three associated plasmids, generated using a combination of the Illumina and PacBio platforms. P. agglomerans ASB05 was isolated from fresh cherries purchased in Albany, CA, in 2016.
Related Concept Videos
Genome Annotation and Assembly
19.6K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
19.6K
Sanger Sequencing
762.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
762.6K

