Related Experiment Video

Updated: Oct 26, 2025

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

909

FOXC2-AS1 stabilizes FOXC2 mRNA via association with NSUN2 in gastric cancer cells

Jijun Yan1, Juntao Liu1, Zhengbin Huang1

  • 1Department of General Surgery, Hanchuan People's Hospital, Hanchuan, 431600, Hubei Province, China.

Human Cell
|July 29, 2021
PubMed

Insights

Long noncoding RNA FOXC2-AS1 promotes gastric cancer by stabilizing FOXC2 mRNA. This oncogenic lncRNA enhances cell proliferation, migration, and invasion, offering a potential therapeutic target for gastric cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNA (lncRNA) FOXC2-AS1 is implicated as an oncogene in various human cancers.
  • The specific role and mechanism of FOXC2-AS1 in gastric cancer (GC) are not well understood.

Purpose of the Study:

  • To investigate the clinical significance, functional role, and molecular mechanism of FOXC2-AS1 in gastric cancer.
  • To determine if FOXC2-AS1 could serve as a therapeutic target for GC.

Main Methods:

  • Quantitative real-time PCR to assess FOXC2-AS1 expression in GC tissues and cells.
  • Cell proliferation, migration, and invasion assays following FOXC2-AS1 knockdown or overexpression.
  • RNA immunoprecipitation and Western blot assays to elucidate the interaction between FOXC2-AS1, FOXC2 mRNA, NSUN2, and YBX1.

Main Results:

  • FOXC2-AS1 expression is significantly upregulated in GC tissues and cells.
  • High FOXC2-AS1 levels correlate with advanced TNM stage and poorer overall survival in GC patients.
  • FOXC2-AS1 knockdown inhibits GC cell proliferation, migration, and invasion, while overexpression promotes these processes.
  • FOXC2-AS1 stabilizes FOXC2 mRNA by recruiting NSUN2, leading to increased m5C modification and YBX1 association.

Conclusions:

  • FOXC2-AS1 functions as an oncogenic lncRNA in gastric cancer by stabilizing FOXC2 mRNA via an m5C-dependent mechanism.
  • FOXC2-AS1 represents a potential novel therapeutic target for gastric cancer treatment.
Keywords:
NSUN2RNA stabilityYBX1m5C modification

More Related Videos

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells09:24

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

Published on: May 9, 2020

4.5K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.5K

Related Concept Videos

Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.3K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.4K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.9K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

3.1K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.9K
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
1.0K