Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.3K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.7K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Explainable Machine-learning Model Based on Multimodal Ultrasound for Non-invasive Detection of Early Renal Fibrosis: A Multicenter Study.

Academic radiology·2026
Same author

Multi-Energy-Driven Photocatalysis: Mechanism, Progress, and Perspective.

Exploration (Beijing, China)·2026
Same author

Tryptamine from wake-active monoaminergic neurons regulates sleep homeostasis.

Nature neuroscience·2026
Same author

Editorial: Two Common Risks, One Greater Harm-Diabetes and Alcohol in Steatotic Liver Disease. Authors' Reply.

Alimentary pharmacology & therapeutics·2026
Same author

Enhanced long-term outcomes with laparoscopic bariatric surgery in patients with severe obesity and metabolic dysfunction-associated steatotic liver disease: a retrospective cohort study.

Hepatobiliary surgery and nutrition·2026
Same author

Multi-Strategy optimisation algorithm for multi-UAV path planning in complex mountainous environments.

Scientific reports·2026

Related Experiment Video

Updated: Oct 26, 2025

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

2.1K

Site-Specific Scissors Based on Myeloperoxidase for Phosphorothioate DNA.

Zhian Hu1, Jinlei Yang1, Fujian Xu2

  • 1Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.

Journal of the American Chemical Society
|July 29, 2021
PubMed
Summary

Researchers discovered a new method for site-specific DNA cleavage using myeloperoxidase (MPO). This endogenous strategy activates DNA devices and enables imaging of oxidative stress in living cells.

More Related Videos

Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

6.8K
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

4.1K

Related Experiment Videos

Last Updated: Oct 26, 2025

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

2.1K
Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

6.8K
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

4.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Site-specific nucleic acid cleavage is crucial for DNA device development.
  • Current methods often rely on external triggers, limiting intracellular applications.
  • Endogenous strategies are needed for studying cellular processes and signaling networks.

Purpose of the Study:

  • To develop an endogenous, site-specific DNA cleavage tool.
  • To investigate the mechanism of myeloperoxidase (MPO) in cleaving phosphorothioate (PT)-modified DNA.
  • To demonstrate the application of this tool in activating DNA machines and imaging intracellular oxidative stress.

Main Methods:

  • Utilized myeloperoxidase (MPO) for site-specific cleavage of phosphorothioate (PT)-modified DNA.
  • Elucidated a scissors-like cleavage mechanism involving chloride oxidation.
  • Applied the MPO-mediated cleavage to activate PT-modified hairpin-DNA machines for DNAzyme production and hybridization chain reaction (HCR) amplification.

Main Results:

  • Accurate site-specific cleavage of PT-modified DNA by MPO was achieved.
  • A mechanism involving MPO-mediated chloride oxidation was revealed.
  • Activated DNA machines successfully produced HRP-mimicking DNAzyme and initiated HCR amplification.
  • HCR amplification enabled robust imaging of oxidative stress in living cells.

Conclusions:

  • An accurate and endogenous site-specific DNA cleavage tool using MPO has been developed.
  • This tool enables the activation of DNA molecular devices and the study of intracellular biostimuli.
  • The system allows for robust imaging of oxidative stress in living cells, highlighting its potential in biological research.