Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

2.2K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
2.2K
Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)01:20

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

1.2K
Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...
1.2K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.2K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.2K
Spin–Spin Coupling: One-Bond Coupling01:17

Spin–Spin Coupling: One-Bond Coupling

1.1K
Coupling interactions are strongest between NMR-active nuclei bonded to each other, where spin information can be transmitted directly through the pair of bonding electrons. While nuclei polarize their electrons to the opposite spins, the bonding electron pair has opposite spins. Configurations with antiparallel nuclear spins are expected to be lower in energy. When coupling makes antiparallel states more favorable, J is considered to have a positive value. The one-bond coupling constant, 1J,...
1.1K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

376
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
376
Spin–Spin Coupling Constant: Overview01:08

Spin–Spin Coupling Constant: Overview

1.1K
In bromoethane, the three methyl protons are coupled to the two methylene protons that are three bonds away. In accordance with the n+1 rule, the signal from the methyl protons is split into three peaks with 1:2:1 relative intensities. The methylene protons appear as a quartet, with the relative intensities of 1:3:3:1.
Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Low Field to High Value: Robust Cortical Mapping From Low-Field MRI.

Human brain mapping·2026
Same author

On the accuracy of image registration in portable low-field 3D brain MRI.

Research square·2026
Same author

Ultra-low field <sup>13</sup>C MRI of hyperpolarized pyruvate.

Communications chemistry·2026
Same author

Enhanced Detection of Acute Ischemic Stroke With Low-Field MRI.

Stroke (Hoboken, N.J.)·2026
Same author

On the accuracy of image registration in portable low-field 3D brain MRI.

bioRxiv : the preprint server for biology·2026
Same author

Breast imaging with ultra-low field MRI.

Scientific reports·2026
Same journal

Spectroscopic Investigation of the In Vivo Light-Dependent Photodynamics of the Marine Diatom Phaeodactylum tricornutum.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Atomistic Insights into the Thermal Decomposition and Runaway Mechanism of Peroxypropionic Acid.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Hydrazine Adsorption on Hexagonal Ice (0001): First-Principles Investigations on Stability, Dynamics, and Chirality Changes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Sustainable Ball Milling-Assisted Synthesis of Bread Waste-Derived Highly Porous Carbons for Adsorption-Based Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

RNALig: An ML-Driven Structure-Based Scoring Function for Estimating Binding Affinities of RNA-Ligand Complexes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Photoswitchable Polar Azobenzene-Based Liquid Crystals for Electro-Optic and Optical Data Storage Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
See all related articles

Related Experiment Video

Updated: Oct 26, 2025

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K

Homonuclear J-Coupling Spectroscopy at Low Magnetic Fields using Spin-Lock Induced Crossing*.

Stephen J DeVience1, Mason Greer2, Soumyajit Mandal3

  • 1Scalar Magnetics, LLC, Windsor Mill, MD 21244, USA.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|July 29, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces spin-lock induced crossing (SLIC), a novel nuclear magnetic resonance (NMR) technique. SLIC enables J-coupling spectra acquisition in low magnetic fields for diverse organic molecules without heteronuclei.

Keywords:
J-couplingNMR spectroscopydressed statelow-field NMRspin-lock induced crossing

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.6K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.3K

Related Experiment Videos

Last Updated: Oct 26, 2025

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.6K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.3K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Background:

  • Nuclear magnetic resonance (NMR) spectroscopy typically necessitates high magnetic fields for spectral resolution.
  • At low fields, strong J-coupling can obscure chemical shift dispersion, resulting in single-line spectra.
  • Existing low-field J-coupling methods often require heteronuclei, limiting their applicability.

Purpose of the Study:

  • To demonstrate a novel pulse sequence for acquiring J-coupling spectra at low magnetic fields.
  • To overcome the limitations of strong-coupling regimes in low-field NMR.
  • To provide a versatile J-coupling spectroscopy method applicable to most organic compounds.

Main Methods:

  • Development and application of a novel pulse sequence termed spin-lock induced crossing (SLIC).
  • SLIC utilizes a weak spin-locking pulse to probe energy level crossings.
  • Spectra acquired at low magnetic fields (276 kHz and 20.8 MHz) for small molecules.

Main Results:

  • SLIC successfully generated unique J-coupling spectra in the strong-coupling regime at low fields.
  • The technique is applicable to a wide range of organic molecules in their native state.
  • Experimental SLIC spectra showed good agreement with simulations.

Conclusions:

  • Spin-lock induced crossing (SLIC) is an effective method for low-field J-coupling spectroscopy.
  • This technique expands the utility of NMR spectroscopy at lower magnetic field strengths.
  • SLIC offers a valuable alternative for analyzing organic molecules without requiring heteronuclei.