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Updated: Oct 26, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

[Construction of tissue engineered cell sheet].

Fuan Xiao¹, Xueting Jian¹, Xiaoyi Feng¹

¹Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, College of Pharmacy, University of South China, Hengyang 421001, Hunan, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|July 30, 2021

Summary

This summary is machine-generated.

Scaffold-free tissue engineered cell sheets offer a promising alternative to traditional methods, avoiding material side effects. This review explores their construction, applications, and future potential in tissue engineering.

Keywords:

cell adhesion cell detachment cell sheet sensitive materials tissue engineering

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Last Updated: Oct 26, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

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Published on: October 20, 2018

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

Area of Science:

Biomedical Engineering
Regenerative Medicine
Tissue Engineering

Background:

Scaffold-free tissue engineered cell sheets are an emerging technology.
They avoid adverse effects associated with scaffold materials.
Cell sheets can be assembled into complex 3D functional tissues.

Purpose of the Study:

To review the construction methods of tissue engineered cell sheets.
To discuss the challenges and future perspectives in this field.

Main Methods:

Cell sheet construction relies on stimuli-responsive culture substrates.
Stimuli include temperature, enzymes, light, ions, redox, pH, and sugar.
Changes in stimuli alter substrate-cell adhesion, enabling natural cell detachment.

Main Results:

Novel stimuli-responsive substrates have led to simple and efficient cell sheet construction technologies.
The resulting cell sheets exhibit excellent performance.
Applications of cell sheets have been significantly expanded.

Conclusions:

Scaffold-free cell sheet technology is advancing rapidly.
Further development is needed to address challenges and realize full potential.
This technology holds significant promise for future biomedical applications.