Influence of sulfur amendments on heavy metals phytoextraction from agricultural contaminated soils: A meta-analysis

Heavy metal pollution is becoming recurrent and threatens biota biosafety in many agricultural fields. Diverse solutions explore the application of amendments to enable remediation. Sulfur represents a nonmetallic chemical element that actively affects heavy metals phytoextraction, and promotes and alternatively mitigates soil functions. In this study, we conduct a meta-analysis to synthesize the current knowledge on the influence of sulfur amendments on plants heavy metals uptake from contaminated soil media. Random-effects model was used to summarize effect sizes from 524 data points extracted from 30 peer reviewed studies. The phytoextraction of cadmium, chromium and nickel were 1.6-, 3.3-, and 12.6-fold, respectively, higher when sulfur amendment was applied; while copper uptake was 0.3-fold lower. Irrespective of the sulfur type, heavy metal extraction increased with the raising sulfur stress. Individual organs showed significant differences of heavy metal uptake between sulfur applied and non-sulfur treatments, and combined organs did not. The heavy metals uptake in leaves and roots were higher in sulfur applied than non-sulfur applied treatments, while those in grain, husk, and stalks were lower. The heavy metals phytoextraction (response ratio) followed the order roots > leaves > stalk > grain > husk. Moreover, heavy metals uptake was 2-fold higher in the sulfur applied than the non-sulfur treatments under ideal (5.5-8) and alkaline conditions (8-14), and 0.2-fold lower under acidic pH (1-5.5). Cadmium, manganese and nickel, and chromium were the most extracted under sulfur application by Vicia sp., Sorghum sp. and Brassica sp., respectively; while chromium, manganese, and iron were the most uptake without sulfur amendments by Oryza sp., Zea sp. and Sorghum sp., respectively. Our study highlights that the influence of sulfur on heavy metal phytoextraction depends on the single or combined effects of sulfur stress intensity, sulfur compounds, plant organ, plant type, and soil pH condition.