Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sputum Studies II: Culture and Sensitivity

Sputum Studies II: Culture and Sensitivity

Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

What we learned from the infection control and what we need in the future: A quantitative and qualitative study on hospital infection prevention and control practitioners (HIPCPs) in Tianjin, China.

American journal of infection control·2024

Same author

Aerosol transmission, an indispensable route of COVID-19 spread: case study of a department-store cluster.

Frontiers of environmental science & engineering·2021

Same author

The global COVID-19 pandemic at a crossroads: relevant countermeasures and ways ahead.

Journal of thoracic disease·2020

Same author

Photoassisted degradation of 2,2',4,4'-tetrabrominated diphenyl ether in simulated soil washing system containing Triton X series surfactants.

Environmental pollution (Barking, Essex : 1987)·2020

Same author

Complete genome analysis of hepatitis B virus in Qinghai-Tibet plateau: the geographical distribution, genetic diversity, and co-existence of HBsAg and anti-HBs antibodies.

Virology journal·2020

Same author

Reducing the number of unnecessary biopsies of US-BI-RADS 4a lesions through a deep learning method for residents-in-training: a cross-sectional study.

BMJ open·2020

Search research articles

Related Experiment Video

Updated: Oct 26, 2025

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

Investigating SARS-CoV-2 persistent contamination in different indoor environments.

He Liu¹, Chunnan Fei¹, Yinglei Chen²

¹Tianjin Centers for Disease Control and Prevention, Tianjin, 300011, PR China.

Environmental Research

|July 30, 2021

Summary

SARS-CoV-2 RNA can persist on surfaces for over 57 days at room temperature and potentially 60 days on cold-chain food packaging. This highlights the risk of environmental contamination and transmission, emphasizing doorknobs and bathrooms as key control points.

Keywords:

COVID-19 Disinfection Low-temperature Persistent contamination SARS-CoV-2

More Related Videos

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Oct 26, 2025

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Published on: January 7, 2019

Area of Science:

Environmental Science
Virology
Public Health

Background:

Environmental contamination by COVID-19 (Coronavirus Disease 2019) poses a transmission risk.
Previous studies focused on short-term SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) contamination on surfaces.

Purpose of the Study:

To investigate the persistence of SARS-CoV-2 RNA in room-temperature and low-temperature environments long after exposure.
To assess the potential for environmental transmission of SARS-CoV-2.

Main Methods:

Collected 11,808 swab samples from a department store, an apartment, cold storages, and food markets.
Analyzed samples for SARS-CoV-2 RNA presence using quantitative methods.
Correlated findings with environmental factors like temperature, humidity, and days since last exposure.

Main Results:

SARS-CoV-2 RNA was detected in room-temperature environments up to 57 days post-exposure (apartment and department store).
Persistent contamination was found on cold storage food packages (23/1360) and surfaces (2/345).
Doorknobs and bathrooms showed significant contamination, with positive rates of 40% and 66.7% respectively in the apartment.

Conclusions:

SARS-CoV-2 RNA can remain detectable for extended periods (at least 57 days) in various environmental settings.
Infectious SARS-CoV-2 may persist on cold-chain food packaging for at least 60 days.
Doorknobs and bathrooms are critical areas for COVID-19 control, and cold-chain logistics workers require enhanced protective measures.