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Published on: June 18, 2013
Intrapartum transfer of oxytocin across the human placenta: An ex vivo perfusion experiment
Nina Olsén Nathan1, Morten Hedegaard2, Gösta Karlsson3
1The Research Unit Women's and Children's Health, The Juliane Marie Centre for Women, Children and Reproduction, Copenhagen University Hospital, Rigshospitalet, Tagensvej 22, 2200, Copenhagen, Denmark; Department of Obstetrics, Copenhagen University Hospital Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark.
Introduction:
Investigation of the maternal to fetal transfer of oxytocin across the dually perfused term human placenta.
Methods:
Human placentae obtained from term singleton pregnancies were utilized in a dual recirculating model of ex vivo placental perfusion. Six placentae from women delivering by elective cesarean at term were perfused, one blank and five with the test substance synthetic oxytocin (0.8 ng/mL) (OX) added to the maternal perfusate for 180 min. Antipyrine was used as positive control to validate overlap of the maternal and fetal circuits. The concentration of OX was determined by radioimmunoassay.
Results:
A fall in maternal concentration of OX was seen throughout the experiment. At 90 min of perfusion a state of equilibrium was reached between maternal and fetal concentrations; however after 180 min the fetal concentration of OX was higher than that of the maternal. 31 % of the test substance was accounted for at the end of the experiment - suggesting OX protein binding and a high degree of oxytocinase activity.
Discussion:
The ex vivo perfusion experiments revealed low transfer of OX to the fetal circuit below physiologically relevant concentrations.
