Effect of key regulators in augmenting transcriptional expression of Transglutaminase in Streptomyces mobaraensis

Transglutaminase forms isopeptide bonds in proteins which are helpful in various industrial applications. However, low productivity and high cost are the major bottlenecks for industrial Transglutaminase production. The present study describes the regulatory mechanism of microbial Transglutaminase (MTGase) biosynthesis from Streptomyces mobaraensis and the effect of key regulators to maximize production. The transcriptional responses under the effect of various key modulators of MTGasebiosynthesis were evaluated. Productivity of MTGase with novel biosynthesis approach by regulators augmentation was correlated by transcriptional profiling. The optimization by key modulators by combinational supplementation led to 2-fold rise in activity. The functional attributes, the copy number of MTGase gene and relative changes were assessed by Real-Time quantitative PCR. Protease, MgCl₂, CTAB induced upregulation, whereas PMSF, NaF and bleomycin sulphate showed inhibitory action on MTGase production and activity. The optimization by combinational supplementation of key modulators led to 4.27-fold increase (6.11 IU/mL) in production.