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Updated: Oct 26, 2025

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Enhancement strategies for photo-fermentative biohydrogen production: A review
Quanguo Zhang1, Shengnan Zhu2, Zhiping Zhang2
1Key Laboratory of New Materials and Facilities for Rural Renewable Energy (MOA of China), Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S&T University, Zhengzhou 450006, China.
Abstract:
Biohydrogen production by photo fermentation is an attractive clean energy production approach with less environmental pollution and higher substrate conversion. In recent years, various measures have been used to improve biohydrogen production performance, but there is a lack of systematic and comprehensive summary and analysis. Hence, the recent literatures on enhancing biohydrogen production by photo fermentation were summarized, and the functional mechanisms of enhancement strategies were explained. In this work, these measures were divided into four categories according to their roles in photo fermentation, including substrate pretreatment, bacterial modification and immobilization, additive addition, reactor design optimization. It can be concluded that the optimal enhancement conditions of each strategy were affected by substrate type, strain and process parameters. According to the results of this work, it was expected to give readers a clear understanding and provide a scientific reference of the research of photosynthetic biohydrogen production.
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