Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The hidden sweet tooth of the black soldier fly (Hermetia illucens).

The Journal of experimental biology·2026

Same author

Effects of nonionic surfactants on life history traits of Drosophila melanogaster.

Environmental science and pollution research international·2025

Same author

Taste evolution in an herbivorous drosophilid.

bioRxiv : the preprint server for biology·2024

Same author

Developmental Differentiations of Major Maize Stemborers Due to Global Warming in Temperate and Tropical Climates.

Insects·2023

Same author

Gustatory function of sensilla chaetica on the labial palps and antennae of three tortricid moths (Lepidoptera: Tortricidae).

Scientific reports·2022

Same author

Interplay between extreme drift and selection intensities favors the fixation of beneficial mutations in selfing maize populations.

Genetics·2021

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Oct 26, 2025

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Published on: May 26, 2023

High-Throughput Feeding Bioassay for Lepidoptera Larvae.

Inoussa Sanané^1,2, Judith Legrand¹, Christine Dillmann¹

¹Université Paris-Saclay, INRAE, CNRS, AgroParisTech, GQE - Le Moulon, 91190, Gif-sur-Yvette, France.

Journal of Chemical Ecology

|July 31, 2021

Summary

A new high-throughput assay screens plant resistance to lepidopteran pests. This system uses automated image analysis to track larval feeding, aiding in the discovery of pest-resistant plants and effective antifeedants.

Keywords:

Digital image analysis Feeding preferences High-throughput device Plant–insect warfare

More Related Videos

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Published on: September 3, 2008

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Published on: June 11, 2020

Related Experiment Videos

Last Updated: Oct 26, 2025

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Published on: May 26, 2023

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Published on: September 3, 2008

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Published on: June 11, 2020

Area of Science:

Agricultural Entomology
Plant Science
Bioinformatics

Background:

Developing pest-resistant plants is time-consuming and expensive.
Lepidopteran pests cause significant crop damage globally.
Efficient screening methods are crucial for agricultural sustainability.

Purpose of the Study:

To present a high-throughput leaf-disk consumption assay for screening plant resistance and chemical antifeedants.
To develop automated image analysis and statistical methods for quantifying larval feeding behavior.
To validate the assay system using azadirachtin and quinine against European corn borer larvae.

Main Methods:

A webcam-based system captures images of larvae feeding on leaf disks over time.
An open-source image analysis program quantifies leaf surface area reduction.
Custom statistical procedures analyze feeding kinetics and treatment effects.

Main Results:

The assay successfully monitored feeding activity of 150 larvae simultaneously.
Azadirachtin demonstrated dose-dependent feeding deterrence.
Quinine showed limited efficacy as an antifeedant within the tested concentration range.

Conclusions:

The developed high-throughput assay is effective for screening plant resistance and chemical antifeedants against lepidopteran pests.
The system provides a cost-effective and efficient alternative to traditional screening methods.
Freely available components facilitate adoption and further development in pest management research.