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Laser-Induced Choroidal Neovascularization in Rats.
Min Zhao1,2, Wankun Xie1,2, Travis W Hein1,2
1Department of Medical Physiology, College of Medicine, Texas A&M University Heath Science Center, Bryan, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 31, 2021
Summary
The laser-induced choroidal neovascularization (CNV) model effectively simulates wet age-related macular degeneration (wet-AMD) for preclinical research. This cost- and time-efficient rodent model allows for standardized treatment evaluation of ocular neovascular diseases.
Area of Science:
- Ophthalmology
- Preclinical Research
- Neovascular Diseases
Background:
- The laser-induced choroidal neovascularization (CNV) model is a standard for studying wet age-related macular degeneration (wet-AMD).
- This model involves laser perforation of the Bruch membrane, inducing neovascularization from choroidal capillaries.
- It is crucial for evaluating therapeutic interventions in ocular neovascular diseases.
Purpose of the Study:
- To detail the protocol for inducing laser-induced CNV in animal models.
- To provide essential notes for successful execution of the CNV induction procedure.
- To establish a reproducible method for preclinical assessment of wet-AMD treatments.
Main Methods:
- Laser application to perforate the Bruch membrane in various species (rat, mouse, pig, monkey).
- Rodent models are preferred for cost and time efficiency.
- The procedure takes 10-15 minutes post-training, with peak CNV at 2 weeks.
Main Results:
- Successful induction of choroidal neovascularization (CNV).
- The model allows for evaluation of treatment efficacy on CNV progression.
- Histologic analysis and fundus imaging are key assessment tools.
Conclusions:
- The laser-induced CNV model is a validated and efficient preclinical tool for wet-AMD research.
- The protocol is adaptable across species, with rodents offering practical advantages.
- This method facilitates the study of ocular neovascular diseases and their treatments.
