Search research articles

Search research articles

Related Experiment Video

Updated: Oct 26, 2025

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Published on: May 23, 2025

Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy.

Samantha J Fredrickson¹, Tanner G Hoog¹, Ryan S Udan²

¹Department of Biology, Missouri State University, Springfield, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 31, 2021

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quencher-Free Fluorescence Monitoring of G-Quadruplex Folding.

ACS omega·2025

Same author

Emergent ribozyme behaviors in oxychlorine brines indicate a unique niche for molecular evolution on Mars.

Nature communications·2024

Same author

FOXO1 represses sprouty 2 and sprouty 4 expression to promote arterial specification and vascular remodeling in the mouse yolk sac.

Development (Cambridge, England)·2022

Same author

DNA G-quadruplexes are uniquely stable in the presence of denaturants and monovalent cations.

Biochemistry and biophysics reports·2022

Same author

Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion.

Chembiochem : a European journal of chemical biology·2022

Same author

Rapid deployment of smartphone-based augmented reality tools for field and online education in structural biology.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2020

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Lightsheet microscopy offers high-resolution imaging with minimal damage, advancing cardiovascular development research. This study presents a new method for visualizing blood vessel development in mouse embryos using this technique.

Area of Science:

Developmental Biology
Microscopy Techniques

Background:

Confocal microscopy faces limitations in resolution and phototoxicity for delicate biological samples.
Advancements in fluorescence microscopy are crucial for detailed studies in developmental biology.
Lightsheet microscopy provides superior optical sectioning and reduced photobleaching.

Purpose of the Study:

To present a novel protocol for preparing and imaging postimplantation mouse embryos.
To demonstrate the utility of lightsheet microscopy for studying cardiovascular development.
To overcome imaging challenges in visualizing intricate blood vessel structures.

Main Methods:

Development of a specific mounting and culturing technique for mouse embryos.
Utilizing lightsheet microscopy for high-resolution, low-phototoxicity imaging.

Keywords:

Angiogenesis Blood vessel Embryo culture Lightsheet microscopy Mouse SPIM Vasculogenesis

More Related Videos

A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

Related Experiment Videos

Last Updated: Oct 26, 2025

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Published on: May 23, 2025

A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

Focusing on the visualization of developing blood vessels within the embryonic context.

Main Results:

Successful high-resolution imaging of blood vessel development in mouse embryos.
Demonstration of minimal photodamage and photobleaching during extended imaging sessions.
Visualization of previously obscured or difficult-to-image vascular structures.

Conclusions:

Lightsheet microscopy is a powerful tool for advancing cardiovascular development research.
The described method enables detailed visualization of embryonic blood vessels.
This technique holds promise for uncovering new insights into heart and vessel formation.