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Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

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Related Experiment Video

Updated: Oct 26, 2025

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Multiplexed Tissue Tomography.

Evan H Phillips¹, David Scholten², Amy C Flor²

¹Department of Pharmaceutical Sciences, The University of Illinois at Chicago, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 31, 2021

Summary

Transparent tissue tomography (T3) allows detailed spatial analysis of markers in whole or thick tissues. This workflow improves upon existing methods for imaging challenging samples like tumors.

Keywords:

Antibody Immunofluorescence Microscopy Multiplex imaging Tissue clearing Tomography

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Highly-Multiplexed Tissue Imaging with Raman Dyes

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Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

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A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

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Published on: March 30, 2020

Area of Science:

Biomedical imaging
Tissue analysis
Spatial biology

Background:

Multiplexed tissue tomography provides spatial insights into tissue markers.
Tissue clearing is essential for deep imaging of thick samples.
Existing tomography methods have limitations with certain tissue types.

Purpose of the Study:

To present a refined workflow for transparent tissue tomography (T3).
To enable comprehensive spatial analysis in difficult-to-clear tissues.
To build upon and improve existing tissue clearing and tomography techniques.

Main Methods:

Detailed workflow for transparent tissue tomography (T3).
Application of clearing agents for tissue transparency.
Deep tissue imaging techniques for spatial marker analysis.

Main Results:

Successful application of T3 in various tissue types.
Demonstrated efficacy in clearing and imaging challenging tissues, including tumors.
Comprehensive spatial mapping of markers within thick tissue sections.

Conclusions:

Transparent tissue tomography (T3) is a robust method for spatial biology.
The T3 workflow enhances imaging capabilities for difficult tissues.
T3 facilitates detailed analysis of marker distribution in whole tissues.