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Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

Lorrane de Andrade Pereira1,2, Margareth Maria de Carvalho Queiroz2, Suzana Côrte-Real Faria3

  • 1Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.

Microscopy Research and Technique
|July 31, 2021
PubMed

Insights

Brevibacillus laterosporus NRS590 effectively controls synanthropic flies, particularly in its sporulation phase. This bacterium shows significant potential for developing microbial insecticides against medically important pests.

Area of Science:

  • Microbial control
  • Entomopathogenic bacteria
  • Insect pest management

Background:

  • Brevibacillus laterosporus exhibits broad entomopathogenic activity, crucial for developing alternatives to synthetic insecticides.
  • Muscoid dipterans pose significant medical, veterinary, and sanitary concerns due to their synanthropic nature.
  • Limited research exists on B. laterosporus efficacy against muscoid dipterans, despite its biotechnological potential.

Purpose of the Study:

  • To evaluate the mortality rates of synanthropic flies caused by Brevibacillus laterosporus NRS590.
  • To conduct ultrastructural characterization of B. laterosporus NRS590 cell stages.

Main Methods:

  • Synanthropic flies were collected and adapted to laboratory conditions.
  • Bioassays utilized B. laterosporus NRS590 from vegetative, sporangium, and free spore phases against fly larvae.
  • Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) were employed for ultrastructural analysis.

Main Results:

  • The sporulation phase of B. laterosporus NRS590 demonstrated high efficacy, achieving 83.3% mortality for Chrysomya megacephala, 85.1% for Chrysomya putoria, and 99% for Musca domestica.
  • Detailed ultrastructural examination revealed the parasporal body on the entire spore surface.
  • The study confirmed the entomopathogenic potential of B. laterosporus against key muscoid dipteran pests.

Conclusions:

  • Brevibacillus laterosporus NRS590, especially in its spore stage, is highly effective against common synanthropic flies.
  • Further research is needed to identify specific virulence factors responsible for its potent entomopathogenic activity.
  • This study supports the development of safe and efficient microbial control strategies for insect pests.
Keywords:
Brevibacillus laterosporus ultrastructureCalliphoridaeentomopathogenicitymicrobial controlmuscidaesynanthropic muscoid dipterans

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