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Related Concept Videos

Green Algae01:21

Green Algae

305
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
305

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Updated: Oct 26, 2025

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
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High-throughput integrated pretreatment strategies to convert high-solid loading microalgae into high-concentration

Geon-Soo Ha1, Shouvik Saha1, Bikram Basak1

  • 1Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, South Korea.

Bioresource Technology
|August 1, 2021
PubMed
Summary

Energy-efficient microwave pretreatment of microalgae enables high-titer biofuel production. This method maximizes biomass utilization and biofuel yields, overcoming energy consumption bottlenecks in microalgal biofuel commercialization.

Keywords:
Cell disruptionHigh titer biofuelIntegrated pretreatmentMicroalgal biorefineryWaste management

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Area of Science:

  • Biotechnology
  • Renewable Energy
  • Chemical Engineering

Background:

  • Commercial biofuel production from microalgae faces challenges due to high energy demands for processing concentrated biomass.
  • Efficiently converting microalgal biomass into biofuels requires overcoming bottlenecks in pretreatment and extraction.

Purpose of the Study:

  • To develop an energy-efficient integrated pretreatment method for high-titer biofuel production from concentrated microalgal biomass.
  • To assess the conversion efficiency and biomass utilization of microalgae using microwave-assisted pretreatment, fermentation, and transesterification.

Main Methods:

  • Utilized microwave pretreatment with low specific energy (4.2 MJ/kg) on 100 g/L microalgal suspensions (Chlamydomonas mexicana and Chlamydomonas pitschmannii).
  • Applied successive fermentation and transesterification processes to convert algal carbohydrates, proteins, and lipids into biofuels.
  • Employed transmission electron microscopy to analyze changes in microalgal cellular integrity post-treatment.

Main Results:

  • Achieved unprecedented total conversion efficiency (67%) and biomass utilization (87%) for Chlamydomonas pitschmannii at 100 g/L.
  • Obtained high yields of bioethanol (0.48 g/g carbohydrates), higher-alcohols (0.44 g/g proteins), and biodiesel (0.90 g/g lipids).
  • Demonstrated significant enhancement in extraction efficiency and overall biomass utilization.

Conclusions:

  • The proposed energy-efficient integrated pretreatment method significantly improves microalgal biofuel production feasibility.
  • This approach minimizes energy consumption and waste production, enabling efficient conversion of high-concentration microalgae.
  • The study highlights a sustainable pathway for producing high-titer biofuels from diverse microalgal feedstocks.