Solid-state anaerobic digestion of sugarcane bagasse at different solid concentrations: Impact of bio augmented...

This study investigated the impact of the potential cellulose degrading bacteria that could be bioaugmented in the solid-state anaerobic digestion (SSAD) of bagasse to enhance the methane yield. The prospective anaerobic cellulose degrading bacteria was isolated from the soil. SSAD experiments were organized with & without bioaugmentation with a substrate total solid (TS) of 25%, 30%, 40% and 50% at an optimized feed to microorganism (F/M) ratio of 1:1. The maximum yield of 0.44 L CH₄/ (g VS added) was obtained from bioaugmented bagasse at a TS of 40% whereas it was 0.34 L CH₄/(g VS added) for non-bioaugmented bagasse. The isolated bacterial strain was identified that belongs species Pseudomonas of Gamma Proteobacteria which exhibited good cellulolytic activity. Metagenomic studies found 90% of archaeal microorganisms affiliated to Methanosaeta, a strict acetoclastic methanogen.