Dynamics of hyperacetylated histone H4 (H4Kac) during spermatogenesis in four decapod crustaceans

During spermatogenesis, the transition from histone to protamine is highly conserved in most invertebrates and vertebrates. Thus far, a large and growing body of literature has demonstrated that histones and histone modifications still exist in the sperm nucleus of decapod crustaceans. H4Kac is believed to play an important role in the process of sperm chromatin condensation. However, the dynamics of hyperacetylated histone H4 (H4Kac) during spermatogenesis in decapoda are still unknown. In this paper, the distribution of H4Kac in four decapod crustaceans (Eriocheir sinensis, Charybdis japonica, Procambarus clarkii, and Macrobrachium nipponense) were investigated via immunofluorescence. Our results indicated that H4Kac was visible in the mature sperm nucleus of E. sinensis, C. japonica, and M. nipponense. Unlike the other three species, H4Kac was translocated from the nuclei to cytoplasm in mid-spermatids of P. clarkii. Eventually, H4Kac were not present in mature spermatozoa of P. clarkii. Importantly, we observed for the first time that H4Kac was distributed outside the nucleus, which reminds us that H4Kac may participate in the formation of acrosome structure in decapod crustaceans and may be a prerequisite for proper chromatin decondensation.