Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neural Regulation01:37

Neural Regulation

40.6K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
40.6K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.4K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.4K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.0K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.0K
Regulated Protein Degradation02:58

Regulated Protein Degradation

2.8K
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Parental Perceived Stress and Its Association With Childhood Obesity in Spain: The PASOS Study.

Preventing chronic disease·2026
Same author

α-Synuclein overexpression increases the tropism of pre-formed fibrils and MSA-patient derived seeds towards oligodendroglia.

Acta neuropathologica communications·2026
Same author

Dynamic uncertainty-level assessment framework for real-time needle tracking in CT-guided surgical environments.

International journal of computer assisted radiology and surgery·2026
Same author

Breastfeeding and dietary habits in childhood and adolescence: the PASOS study.

International breastfeeding journal·2026
Same author

Real-time marker-less needle tracking for CT-guided interventions using multiple RGB cameras.

International journal of computer assisted radiology and surgery·2026
Same author

Single-nucleus brain transcriptomics reveals microglia dysfunction in multiple system atrophy.

Nature communications·2026

Related Experiment Video

Updated: Oct 26, 2025

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

6.9K

DNAJB6b is Downregulated in Synucleinopathies.

Jonas Folke1, Sertan Arkan2, Isak Martinsson3

  • 1Research Laboratory for Stereology and Neuroscience, Bispebjerg-Frederiksberg Hospital, University Hospital of Copenhagen, Copenhagen, Denmark.

Journal of Parkinson'S Disease
|August 2, 2021
PubMed
Summary

The co-chaperone DNAJB6b, a suppressor of alpha-synuclein aggregation, is downregulated in neurodegenerative diseases like Parkinson's. This finding highlights DNAJB6b as a potential therapeutic target for these conditions.

Keywords:
Alpha-synucleinDNAJB6clinical samplesneurodegenerationsynucleinopathy

More Related Videos

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
09:27

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

Published on: January 5, 2016

17.7K
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

24.0K

Related Experiment Videos

Last Updated: Oct 26, 2025

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

6.9K
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
09:27

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

Published on: January 5, 2016

17.7K
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

24.0K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Alpha-synuclein aggregation is implicated in neurodegenerative disease progression.
  • DNAJB6 isoform b (DNAJB6b) suppresses alpha-synuclein aggregation.
  • The role of endogenous DNAJB6b in health and disease is unknown due to a lack of specific antibodies.

Purpose of the Study:

  • Generate a novel anti-DNAJB6b antibody.
  • Analyze the cellular and tissue localization of DNAJB6b.
  • Investigate DNAJB6b expression levels in neurodegenerative diseases.

Main Methods:

  • Immunocytochemistry and immunohistochemistry for localization.
  • Quantitative DNAJB6 specific ELISA for expression analysis.
  • Analysis of human brain tissue from patients with Parkinson's disease, multiple system atrophy, and progressive supranuclear palsy.

Main Results:

  • Endogenous DNAJB6b is primarily localized in the cytoplasm and neurites (dendrites > axons) in vitro.
  • DNAJB6b is expressed in dopaminergic neurons of the substantia nigra pars compacta in vivo.
  • DNAJB6b levels are downregulated in Parkinson's disease, multiple system atrophy, and progressive supranuclear palsy brain tissue.
  • Total DNAJB6 levels are upregulated in these diseases, suggesting potential compensatory upregulation of DNAJB6a.

Conclusions:

  • DNAJB6b is downregulated in multiple neurodegenerative diseases.
  • DNAJB6b represents a potential therapeutic target for investigating amyloid protein aggregation and disease progression.
  • Further research into DNAJB6b's role in neurodegeneration is warranted.