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Bovine antibody dependent cell-mediated cytotoxicity (ADCC) effector cells
1Department of Molecular Biology, College of Agriculture, University of Wyoming, Laramie 82071.
Veterinary Immunology and Immunopathology
|November 1, 1987
Summary
Bovine immune cells, including neutrophils and monocytes, were analyzed for antibody-dependent cell-mediated cytotoxicity (ADCC). Results confirm the presence of ADCC-active null lymphocytes in cattle, with varying cell activity depending on the sensitizing antisera used.
Area of Science:
- Immunology
- Cell Biology
- Veterinary Science
Background:
- Antibody-dependent cell-mediated cytotoxicity (ADCC) is a crucial immune mechanism.
- Identifying effector cells involved in ADCC in bovine species is important for understanding cattle immunity.
Purpose of the Study:
- To characterize the ADCC-mediating capacity of different bovine peripheral blood cell populations.
- To investigate the role of neutrophils, monocytes, and null lymphocytes in ADCC.
Main Methods:
- Bovine blood cells were isolated using centrifugation, adherence, and rosetting techniques.
- Enriched populations of peripheral blood mononuclear cells (PBM), null lymphocytes, monocytes, and neutrophils were tested for ADCC activity.
- Both heterologous and homologous sensitizing antisera were employed to evaluate cell-mediated cytotoxicity.
Main Results:
- Monocytes exhibited approximately twice the ADCC activity of null lymphocytes when using heterologous antisera.
- Neutrophils showed minimal ADCC activity with heterologous antisera but possessed the greatest activity with homologous antisera.
- Neutrophils, monocytes, and null cells displayed significant ADCC activity with homologous antisera, in descending order.
Conclusions:
- The study confirms the existence of ADCC-active null lymphocytes in bovine peripheral blood.
- Neutrophils and monocytes are significant contributors to ADCC in cattle, with their activity being dependent on the type of sensitizing antibody.