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Updated: Oct 26, 2025
Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
Published on: March 16, 2016
Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
Shanshan Cheng1, Yuqian Shen1, Zi Chao Zhang1,2
1School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China.
Abstract:
Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an interfering peptide into the adult mouse hippocampus. The delivered peptide disrupts the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2, resulting in impaired hippocampal mGluR-LTD and mGluR-LTD-associated behaviors. For complete details on the use and execution of this protocol, please refer to Shen et al. (2021).
Insights
This study details a method to disrupt hippocampal metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) in mice. The technique impairs mGluR-LTD and associated learning and memory behaviors.
Area of Science:
- Neuroscience
- Molecular Biology
Background:
- Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) is crucial for neural plasticity, behavior, learning, and memory.
- mGluR-LTD requires rapid, local protein synthesis within minutes.
Purpose of the Study:
- To present a protocol for disrupting mGluR-LTD in the adult mouse hippocampus.
- To investigate the role of specific protein interactions in mGluR-LTD.
Main Methods:
- A protocol for delivering an interfering peptide into the adult mouse hippocampus was developed.
- The peptide was designed to disrupt the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2.
Main Results:
- The delivered peptide successfully impaired hippocampal mGluR-LTD.
- Disruption of mGluR-LTD led to deficits in associated behaviors.
Conclusions:
- The described method effectively inhibits mGluR-LTD in adult mice.
- Targeting the polyglutamine binding protein 1 and eukaryotic elongation factor 2 interaction is a viable strategy to modulate synaptic plasticity and behavior.
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