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[Changes in erythrocyte deformability in blood preservation].
Zeitschrift Fur Die Gesamte Innere Medizin Und Ihre Grenzgebiete
|October 15, 1987
Summary
Erythrocyte storage in the GDR using CDS-AG medium caused significant deformability loss, impacting transfusion effectiveness. A new resuspension medium now prevents this, improving erythrocyte quality for transfusions.
Area of Science:
- Biomedical Engineering
- Hematology
- Blood Storage
Background:
- Erythrocyte deformability is crucial for blood circulation and oxygen delivery.
- Storage conditions significantly impact erythrocyte integrity and function.
- Previous erythrocyte storage methods in the GDR led to suboptimal outcomes.
Purpose of the Study:
- To characterize erythrocyte deformability changes during storage in the GDR.
- To identify the causes of deformability loss in specific storage media.
- To develop an improved resuspension medium to maintain erythrocyte deformability.
Main Methods:
- Filtration procedures were used to assess erythrocyte deformability.
- Cryopreservation at -196°C and -28°C were compared.
- Erythrocyte concentrates were resuspended in CDS-AG and a new medium.
- In vivo survival studies were conducted.
Main Results:
- CDS-AG resuspension caused a 75% loss in erythrocyte deformability due to increased viscosity and irreversible shrinkage.
- In vivo survival rates for CDS-AG resuspended erythrocytes were below the acceptable limit.
- CPDA-1 and SAGM resuspensions showed minimal deformability changes.
- The new GDR resuspension medium prevented drastic deformability loss, performing comparably to SAGM.
Conclusions:
- The low ionic strength of CDS-AG medium is detrimental to erythrocyte deformability.
- A modified resuspension medium effectively preserves erythrocyte deformability during storage.
- Improved storage solutions are essential for effective erythrocyte transfusions.