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Related Experiment Video

Updated: Oct 26, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy.

Tilman Franke1, Irina Kolotuev2

  • 1Thermo Fisher.

Journal of Visualized Experiments : Jove
|August 2, 2021
PubMed
Summary

Lateral screening with Array Tomography in Scanning Electron Microscopy (SEM) improves cell biology imaging. This method efficiently localizes and images specific cellular areas, overcoming limitations of traditional techniques.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Nanotechnology

Background:

  • Electron microscopy, including Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), is crucial for high-resolution imaging in biology and medicine.
  • While TEM offers superior resolution for protein-level details, SEM is sufficient for many organelle-level investigations.
  • Advanced SEM methods like Serial Block-Face SEM (SBF-SEM) and Focused Ion Beam SEM (FIB-SEM) enable volume acquisition but struggle with precise localization of target areas.

Purpose of the Study:

  • To introduce and validate a novel
  • lateral screening
  • strategy using Array Tomography in SEM.
  • To enhance the efficiency of identifying and navigating to specific areas of interest within biological samples for high-resolution imaging.
  • To overcome the data acquisition inefficiencies and sample preparation challenges associated with current advanced electron microscopy techniques.

Main Methods:

  • Development and application of a
  • lateral screening
  • workflow integrating Array Tomography with SEM.
  • Utilizing Array Tomography to create section libraries of conserved samples for repeated imaging.
  • Implementing automated imaging protocols for selected regions of interest identified through lateral screening.

Main Results:

  • Demonstrated successful localization of challenging-to-access structural details using the lateral screening approach.
  • Showcased the ability to precisely target and image relevant fractions of the total sample volume, reducing unnecessary data acquisition.
  • Array Tomography samples proved amenable to repeated imaging, facilitating comprehensive analysis.

Conclusions:

  • Lateral screening with Array Tomography in SEM significantly improves the efficiency and precision of cellular and structural imaging.
  • This strategy provides a powerful solution for analyzing intricate biological structures that are difficult to study with conventional methods.
  • The method enables targeted high-resolution analysis, optimizing the use of advanced electron microscopy techniques in cell biology research.