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The effectiveness of extended binding affinity of prophage lysin PlyARI against Streptococcus suis infection

Yuyi Xiao1,2,3, Rong Chen4, Min Li1,2,3

  • 1OIE Reference Lab for Swine Streptococcosis, Nanjing, 210095, China.

Archives of Microbiology
|August 2, 2021
PubMed

Insights

A novel lysin, PlyARI, shows potent antibacterial activity against drug-resistant Streptococcus suis. This potential new agent effectively killed S. suis in vitro and protected mice from infection, offering hope for new treatments.

Area of Science:

  • Microbiology
  • Bacteriology
  • Antimicrobial Research

Background:

  • Streptococcus suis is a significant zoonotic pathogen causing severe infections.
  • Increasing multi-drug resistance in S. suis necessitates novel therapeutic strategies.
  • Traditional antibiotics are becoming less effective against resistant strains.

Purpose of the Study:

  • To identify and characterize a novel antibacterial agent against Streptococcus suis.
  • To evaluate the efficacy and specificity of the lysin PlyARI.
  • To explore PlyARI as a potential alternative to conventional antibiotics.

Main Methods:

  • Recombinant expression and purification of lysin PlyARI from prophage PhiARI0460-1.
  • In vitro assessment of bacteriolytic activity, minimum inhibitory concentration (MIC), and pH stability.
  • In vivo efficacy testing using a mouse infection model.
  • Evaluation of binding specificity using a green fluorescent protein (GFP) fusion protein.

Main Results:

  • Recombinant PlyARI demonstrated high bacteriolytic activity against 30 S. suis isolates with an MIC of 2 µg/mL.
  • PlyARI maintained lytic efficiency across a broad pH range (4-12).
  • A dose of 0.5 mg PlyARI provided complete protection in a mouse model challenged with virulent S. suis.
  • PlyARI exhibited high binding affinity for S. suis, Staphylococcus aureus, and Streptococcus equi.

Conclusions:

  • PlyARI is a potent antibacterial agent effective against Streptococcus suis.
  • Its broad pH stability and in vivo efficacy suggest therapeutic potential.
  • PlyARI shows promise as an alternative antibacterial agent for treating and preventing Streptococcal infections.
Keywords:
Antibacterial agentsPlyARISH3b domainStreptococcus suis

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