Colorimetric and Ratiometric Fluorescence Detection of HSO₃^- With a NIR Fluorescent Dye

Area of Science:

• Analytical Chemistry
• Biochemistry
• Chemical Sensing

Background:

• Bisulfite (HSO3-) is extensively used in food and industry.
• Widespread bisulfite use poses significant risks to human health.
• There is a need for effective methods to detect and monitor bisulfite levels.

Purpose of the Study:

• To design and synthesize a novel probe (PBI) for bisulfite detection.
• To develop a selective and quantitative method for bisulfite sensing.
• To enable visual identification and cellular imaging of bisulfite.

Main Methods:

• Synthesis of the PBI probe.
• Investigation of the nucleophilic addition reaction between PBI and bisulfite.
• Spectrophotometric analysis for quantitative detection.
• Fluorescence imaging in living cells.

Main Results:

• The PBI probe demonstrated selective reactivity towards bisulfite.
• Quantitative detection of bisulfite was achieved using the PBI probe.
• A distinct color change in PBI allowed for naked-eye identification of bisulfite.
• Successful application of PBI for fluorescence imaging of bisulfite in living cells.

Conclusions:

• The PBI probe offers a selective, quantitative, and visually detectable method for bisulfite.
• This probe provides a valuable tool for bisulfite detection in various applications, including biological imaging.
• The developed method simplifies bisulfite monitoring and enhances health safety assessments.