Related Experiment Video
Updated: Oct 26, 2025
10:07
Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
24.2K
Evolutionarily distinct resistance proteins detect a pathogen effector through its association with different host
Haixia Wang1,2, Franziska Trusch1, Dionne Turnbull1
1Division of Plant Sciences, University of Dundee, At James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.
The New Phytologist
|August 2, 2021
Summary
Plant immune receptors R2 and Rpi-mcq1 recognize the pathogen effector PiAVR2 through distinct mechanisms involving different BSL phosphatases, crucial for durable disease resistance.
Area of Science:
- Plant pathology
- Molecular biology
- Evolutionary biology
Background:
- Understanding pathogen recognition mechanisms is key to developing durable disease resistance in plants.
- Phytophthora infestans effector PiAVR2 is recognized by distinct plant resistance proteins, R2 and Rpi-mcq1.
Purpose of the Study:
- To investigate how evolutionarily distinct resistance proteins R2 and Rpi-mcq1 recognize the P. infestans effector PiAVR2.
- To determine the role of BSL phosphatases in mediating R2- and Rpi-mcq1-triggered hypersensitive responses (HR).
Main Methods:
- Yeast two-hybrid assays
- Co-immunoprecipitation
- Virus-induced gene silencing
- Transient overexpression
- Phosphatase activity assays
Main Results:
- BSL1 activity and association with R2 are essential for R2-mediated recognition of PiAVR2.
- BSL2 and BSL3 activity mediate Rpi-mcq1 recognition of PiAVR2.
- Distinct BSL phosphatases are involved in the recognition of PiAVR2 by R2 and Rpi-mcq1, highlighting their central role in plant immunity.
Conclusions:
- R2 and Rpi-mcq1 employ different strategies to detect the effector PiAVR2, utilizing distinct BSL phosphatases.
- These findings reveal crucial insights into the molecular basis of plant disease resistance and pathogen recognition evolution.
Related Concept Videos
01:31Defense Against Bacterial Pathogens
1.7K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.7K
01:16Transduction
372
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
372
01:28Diversity of Antigen Receptors
998
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
998
00:42Cross-reactivity
31.8K
Overview
31.8K
02:26Defenses Against Pathogens and Herbivores
28.1K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
28.1K
01:57Protein Complexes with Interchangeable Parts
2.7K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.7K