Molybdate effectively controls sulphide production in a shrimp pond model

The production of shrimp is often performed in earthen outdoor ponds in which the high input of feed and faeces on the bottom can result in deterioration of the water quality, which negatively impacts the animals and the environment. Here, we investigate the potential of sodium molybdate (Na₂MoO₄·2H₂O), sodium nitrate (NaNO₃) and sodium percarbonate (Na₂CO₃·1.5H₂O₂) to control sulphide production in a simulated shrimp pond bottom system that included the sediment, overlaying artificial seawater and organic matter input in the form of shrimp feed and shrimp faeces. Sediment depth gradient measurements of oxygen, H₂S and pH were obtained during 7 days of incubation using microelectrodes. The most significant impact in terms of H₂S, was observed for 50 mg/L sodium molybdate. At the water-sediment interface, there was up to 73% less H₂S detected for this treatment in comparison to a control treatment, while in the deeper layers of the sediment it was up to 47% less H₂S. The residual sulphate concentrations in the molybdate treated samples were 16 ± 4% higher than the control, indicating an inhibition in sulphate reduction. Nitrate and sodium percarbonate treatments also showed a limited capacity to decrease H₂S entering in the water column, yet no clear difference in H₂S concentrations in the sediment compared to the control were observed. Molybdate treatment appears to work through the inhibition of sulphate reducing bacteria in situ for the control of H₂S production better than the chemical oxygen boosters or nitrate treatment.