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Proteomics

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Related Experiment Video

Updated: Oct 26, 2025

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Decoding Post-Translational Modification Crosstalk With Proteomics.

Mario Leutert¹, Samuel W Entwisle², Judit Villén²

¹Department of Genome Sciences, University of Washington, Seattle, Washington, USA.

Molecular & Cellular Proteomics : MCP

|August 2, 2021

Summary

This summary is machine-generated.

Post-translational modifications (PTMs) regulate cellular functions and protein complexity. This review covers how advanced proteomic technologies enable the study of PTM crosstalk, revealing complex regulatory networks.

Keywords:

crosstalk mass spectrometry post-translational modification proteomics

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Last Updated: Oct 26, 2025

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Post-translational modifications (PTMs) are crucial for protein function regulation, signal transduction, and cellular responses.
PTMs increase proteome complexity by expanding protein functionality and diversity.
PTM crosstalk involves the combinatorial action of multiple PTMs for higher-order cellular regulation.

Purpose of the Study:

To review recent advances in proteomic technologies, mass spectrometry, and bioinformatics for identifying PTM crosstalk.
To provide an overview of PTM crosstalk modes, elucidation methods, and functional consequence analysis.

Main Methods:

Leveraging advances in high-throughput proteomic technologies.
Utilizing sophisticated mass spectrometry instrumentation for PTM site identification.
Applying bioinformatics approaches for proteome-wide PTM crosstalk analysis.

Main Results:

Proteomic technologies have spurred the identification of PTM crosstalk across the proteome.
Measurements of PTM sites are key to elucidating these complex interactions.
The review consolidates knowledge on PTM crosstalk mechanisms and detection.

Conclusions:

Advanced technologies are essential for understanding the intricate network of PTM crosstalk.
Elucidating PTM crosstalk provides insights into higher-order protein regulation.
Future research can build upon these methods to explore functional consequences.