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Updated: Oct 26, 2025

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Improving stability of mainstream Anammox in an innovative two-stage process for advanced nitrogen removal from
Fangzhai Zhang1, Yongzhen Peng2, Yongwang Liu3
1China Architecture Design and Research Group, Beijing 100044, PR China; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Abstract:
This study presents an innovative mainstream Anammox based on multiple NO2--N supplement pathways to treat actual mature landfill leachate over 180 days. Desirable effluent quality of 11.8 mg/L total nitrogen (TN) and nitrogen removal efficiency of 98.8% were achieved despite fluctuation conditions of 1.5-fold influent substrates and 8.0-fold dissolved oxygen overload. Nitrogen mass balance confirmed Anammox was the dominant nitrogen removal pathway, contributing up to 87.9%. Functional genes of ammonia monooxygenase (amoA), hydrazine synthase (hzsB), and ratio of nitrate/nitrite reductase were highly detected. Anammox genera, Candidatus_Kuenenia (4.1%) and Candidatus_Brocadia (5.3%) were dominant in two functional systems, respectively, due to the different affinity of nitrite, oxygen, and organic carbon. As an economical and sustainable technology, the innovative process enabled a 95.1% decrease in organic carbon demand, a 61.5% reduction in aeration energy consumption, and 77.6% lower biomass production compared with traditional nitrification-denitrification process.
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