Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.6K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.6K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.7K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

13.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
13.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting in vivo performance of piroxicam and apalutamide drug nanocrystals from in vitro flux measurements combined with in silico modelling.

International journal of pharmaceutics·2026
Same author

Effect of HIFU frequency on gold removal efficiency from e-waste.

Scientific reports·2026
Same author

Correction to "Effect of Ultrasound Standing Wave-Induced Acoustophoresis in Monoglyceride Oleogel Structuration".

Crystal growth & design·2026
Same author

High-power burst and chirp amplifier for MHz ultrasonics.

The Review of scientific instruments·2025
Same author

Use of Prussian Blue pseudocapacitive properties to amplify the pulsed amperometric readout of biosensors.

Biosensors & bioelectronics·2025
Same author

Wireless trigger distribution with nanosecond jitter based on ultra-wideband transceiver modules.

HardwareX·2025
Same journal

A compact low-power magnetic particle imaging scanner based on a permanent-magnet field-free-line generator with high gradient.

The Review of scientific instruments·2026
Same journal

Achieving ultrahigh resolution with high efficiency: Optical design of the two-dimensional Resonant Inelastic X-ray Scattering (2D-RIXS) spectrometer at NanoTerasu beamline 02U.

The Review of scientific instruments·2026
Same journal

Automated laboratory x-ray diffractometer and fluorescence spectrometer for high-throughput materials characterization.

The Review of scientific instruments·2026
Same journal

Nonlinear Bayesian Doppler tomography for simultaneous reconstruction of flow and temperature.

The Review of scientific instruments·2026
Same journal

A Reflectance-based multimodal wearable photoplethysmography (PPG) sensor.

The Review of scientific instruments·2026
Same journal

Temporal analysis of products-Raman (TAP-Raman): An integrated setup for operando spectroscopy and transient kinetic analysis.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Oct 26, 2025

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

14.2K

CESAM-Coded excitation scanning acoustic microscope.

Antti Meriläinen1, Jere Hyvönen1, Ari Salmi1

  • 1Electronics Research Laboratory, Department of Physics, University of Helsinki, Gustaf Hällströmin katu 2, FIN 00560 Helsinki, Finland.

The Review of Scientific Instruments
|August 3, 2021
PubMed
Summary
This summary is machine-generated.

Coded Excitation Scanning Acoustic Microscopy (CESAM) uses coded signals to significantly improve image quality and speed. This advanced technique enhances signal-to-noise ratios, making acoustic microscopy more effective for various applications.

More Related Videos

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

2.7K
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
09:31

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

3.2K

Related Experiment Videos

Last Updated: Oct 26, 2025

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

14.2K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

2.7K
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
09:31

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

3.2K

Area of Science:

  • Materials Science
  • Biology
  • Industrial Quality Control

Background:

  • Scanning Acoustic Microscopy (SAM) offers high resolution and mechanical property quantification.
  • Traditional SAM imaging can be slow, particularly when signal averaging is required.

Purpose of the Study:

  • To introduce a Coded Excitation Scanning Acoustic Microscope (CESAM) for faster and higher-quality acoustic imaging.
  • To demonstrate the benefits of using coded signals with linear and non-linear frequency modulation in acoustic microscopy.

Main Methods:

  • Implementation of coded excitation signals instead of short bursts in SAM.
  • Utilizing both linear and non-linear frequency modulation techniques.
  • Comparison of CESAM performance against traditional SAM in terms of Signal-to-Noise Ratio (SNR) and echo duration.

Main Results:

  • CESAM with linear chirp increased SNR by 16.3 dB and reduced echo duration by 26.7% (130-370 MHz).
  • CESAM with non-linear chirps achieved a 10.3 dB SNR increase and 70.5% echo duration reduction (100-450 MHz).
  • Shorter echo duration improves axial (z) resolution, while lateral resolution remains diffraction-limited.

Conclusions:

  • Coded excitation significantly enhances image quality (SNR, contrast-to-noise ratio) in acoustic microscopy.
  • CESAM offers a viable method to improve imaging speed and quality without compromising measurement time.
  • This advancement holds potential to revitalize acoustic microscopy applications, especially for challenging samples.