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Related Experiment Video

Updated: Oct 26, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Circular RNA translation, a path to hidden proteome.

Tanvi Sinha¹, Chirag Panigrahi¹, Debojyoti Das^1,2

¹Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, India.

Wiley Interdisciplinary Reviews. RNA

|August 3, 2021

Summary

Circular RNAs (circRNAs) can translate into proteins, expanding the known proteome. This review explores circRNA translation mechanisms and the roles of these circ-proteins in health and disease.

Keywords:

IRES cap-independent translation circRNA m6A polypeptide

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Last Updated: Oct 26, 2025

Identification of Circular RNAs using RNA Sequencing

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Published on: November 14, 2019

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Area of Science:

Molecular Biology
Genomics
Proteomics

Background:

Messenger RNA (mRNA) typically encodes proteins, but noncoding RNAs, including circular RNAs (circRNAs), are more abundant.
circRNAs are increasingly recognized for their roles in gene regulation and, notably, their potential to produce proteins.
Features like Open Reading Frames (ORFs) and internal ribosomal entry sites (IRES) suggest circRNAs can initiate cap-independent translation.

Purpose of the Study:

To review the mechanisms underlying circRNA translation.
To highlight the significance of circRNA-encoded proteins (circ-proteins) in cellular functions and disease states.
To discuss methods for identifying translatable circRNAs and characterizing circ-proteins.

Main Methods:

Computational approaches for identifying circRNAs with coding potential.
Molecular biology techniques for validating circRNA translation.
Functional assays to characterize the roles of circ-proteins.

Main Results:

circRNAs possess features enabling cap-independent translation.
circ-proteins are involved in various cellular processes and disease pathologies.
Systematic identification and characterization of circ-proteins are advancing.

Conclusions:

circRNA translation represents a novel source of proteins, contributing to the cellular proteome.
Understanding circ-protein function is crucial for deciphering cellular physiology and disease.
Future research on circRNA translation holds promise for therapeutic applications.