Disruption of AtHAK/KT/KUP9 enhances plant cesium accumulation under low potassium supply

Understanding the molecular mechanisms that underlie cesium (Cs⁺ ) transport in plants is important to limit the entry of its radioisotopes from contaminated areas into the food chain. The potentially toxic element Cs⁺ , which is not involved in any biological process, is chemically closed to the macronutrient potassium (K⁺ ). Among the multiple K⁺ carriers, the high-affinity K⁺ transporters family HAK/KT/KUP is thought to be relevant in mediating opportunistic Cs⁺ transport. Of the 13 KUP identified in A. thaliana, only HAK5, the major contributor to root K⁺ acquisition under low K⁺ supply, has been functionally demonstrated to be involved in Cs⁺ uptake in planta. In the present study, we showed that accumulation of Cs⁺ increased by up to 30% in two A. thaliana mutant lines lacking KUP9 and grown under low K⁺ supply. Since further experiments revealed that Cs⁺ release from contaminated plants to the external medium is proportionally lower in the two kup9 mutant alleles, we proposed that KUP9 disruption could impair Cs⁺ efflux. By contrast, K⁺ status in kup9 mutants is not affected, suggesting that KUP9 disruption does not alter substantially K⁺ transport in experimental conditions used. The putative primary role of KUP9 in plants is further discussed.