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Related Experiment Video

Updated: Oct 26, 2025

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
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PRISM: A Novel Human Islet Isolation Technique.

Jason B Doppenberg1,2, Marten A Engelse1, Eelco J P de Koning1

  • 1Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands.

Transplantation
|August 3, 2021
PubMed
Summary

A new closed system, the PancReatic Islet Separation Method (PRISM), simplifies pancreatic islet isolation. This method improves efficiency and requires fewer operators for islet transplantation in type 1 diabetes.

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Area of Science:

  • Endocrinology
  • Transplantation Biology
  • Medical Device Development

Background:

  • Pancreatic islet isolation is crucial for type 1 diabetes transplantation but is complex and manual.
  • Simplifying islet isolation can enhance yield, quality, and reduce costs.
  • Current methods require significant operator time and resources.

Purpose of the Study:

  • To develop and evaluate a novel, closed system for pancreatic islet isolation.
  • To simplify the islet isolation procedure and improve efficiency.
  • To assess the yield and quality of islets isolated using the new method.

Main Methods:

  • Developed the PancReatic Islet Separation Method (PRISM), a closed system for tissue processing.
  • Optimized pump and centrifuge parameters using microspheres.
  • Applied PRISM to isolate islets from 10 human pancreases.

Main Results:

  • PRISM isolation can be performed by a single operator in one flow cabinet.
  • Islet yield was comparable to traditional methods (431,234 ± 292,833 vs. 285,276 ± 197,392 islet equivalents).
  • PRISM-isolated islets demonstrated similar viability, morphology, and function (glucose-stimulated insulin secretion).

Conclusions:

  • PRISM offers a novel, efficient technique for pancreatic islet isolation.
  • The method significantly improves islet isolation efficiency with reduced operator requirements.
  • PRISM has the potential to streamline islet isolation for transplantation.