Patterns of heart rate and cardiac pausing in unrestrained resting decapod crustaceans

Measurement of heart rate (HR) has been used as an important physiological indicator in a broad range of taxa. In the present study HR patterns were measured in five species of unrestrained, resting decapod crustaceans. In addition to variation in HR among individuals, it was also very variable within an individual animal. While some of this variation was related to activity, there was also a non-locomotory component. Unstressed, resting crabs exhibited intermittent heart activity, whereas HR in stressed crabs was more stable, suggesting differential control of HR in resting crabs. Once the animals settled in the experimental apparatus they exhibited regular and extended cardiac pauses (acardia) of 15-300-s duration. As with HR, there was a significant variation in the frequency and length of acardic events, which were only observed in inactive crabs. Regaining of HR, following a period of acardia, was characterized by small adjustments in position and movement of the mouthparts. This rhythmic pattern, and the fact that entry into and out of acardia was not instantaneous, suggested that these events were related to release of neurohormones and their subsequent degradation in the system, rather than direct neural control of the heart. Because HR was variable and interrupted by regular periods of acardia, caution is recommended when calculating baseline levels of HR, or using HR alone as an indicator of physiological stress. Incorporating a coefficient of variation for HR and/or measuring the periods of acardia may be a more reliable indicator of physiological stress in decapod crustaceans.