Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.1K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.1K
Urine Studies I: Urinalysis01:29

Urine Studies I: Urinalysis

448
Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
448

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving the Electrocatalytic Activity and Durability of the La<sub>0.6</sub>Sr<sub>0.4</sub>Co<sub>0.2</sub>Fe<sub>0.8</sub>O<sub>3-δ</sub> Cathode by Surface Modification.

ACS applied materials & interfaces·2018
Same author

Oriented electron transmission in polyoxometalate-metalloporphyrin organic framework for highly selective electroreduction of CO<sub>2</sub>.

Nature communications·2018
Same author

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Cell·2018
Same author

Impact of Strain-Induced Changes in Defect Chemistry on Catalytic Activity of Nd<sub>2</sub>NiO<sub>4+δ</sub> Electrodes.

ACS applied materials & interfaces·2018
Same author

Curcumin induces apoptosis and inhibits angiogenesis in murine malignant mesothelioma.

International journal of oncology·2018
Same author

Deletion of SMARCA4 impairs alveolar epithelial type II cells proliferation and aggravates pulmonary fibrosis in mice.

Genes & diseases·2018

Related Experiment Video

Updated: Oct 25, 2025

Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.6K

Integrated hand-held electrochemical sensor for multicomponent detection in urine.

Jiang Liu1, Wei Lu1, Lu Zhang1

  • 1Flexible Printed Electronics Technology Center and School of Science, Harbin Institute of Technology, Shenzhen, Guangdong, 518055, China.

Biosensors & Bioelectronics
|August 3, 2021
PubMed
Summary

A novel tip-like electrochemical sensor prototype simplifies multi-analyte detection in point-of-care testing (POCT). This sensor integrates with a pipettor for semi-automatic analysis, detecting uric acid, urea, and pH in urine samples with high accuracy.

Keywords:
Electrochemical sensorNovel sensing strategyPoint-of-care testingSimultaneous detectionUrine

More Related Videos

Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing
06:55

Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing

Published on: May 8, 2021

5.8K
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.0K

Related Experiment Videos

Last Updated: Oct 25, 2025

Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.6K
Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing
06:55

Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing

Published on: May 8, 2021

5.8K
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.0K

Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Biosensors

Background:

  • Electrochemical sensors offer potential for point-of-care testing (POCT) but face challenges like sample interference, limited multi-analyte detection, and complex procedures.
  • Existing sensors often require modifications and struggle with simultaneous analysis of multiple targets in complex matrices like urine.

Purpose of the Study:

  • To develop a simple, tip-like electrochemical sensor prototype for semi-automatic, multi-analyte detection in POCT.
  • To establish a novel sensing strategy utilizing multiple electrochemical signals for simultaneous analysis of uric acid, urea, and pH.
  • To mitigate interference from sample pH variations in electrochemical assays.

Main Methods:

  • Fabrication of a tip-like electrochemical sensor without additional bio/chem modifications.
  • Integration of the sensor with a commercial pipettor for semi-automatic sampling, detection, and rinsing.
  • Development of a multi-component sensing strategy using open circuit potential, peak current, and specific reaction potentials.
  • Calibration using pH-related parameters to reduce interference from sample pH variations.

Main Results:

  • The tip sensor achieved semi-automatic assay procedures, reducing operational time and manual effort.
  • Simultaneous detection of uric acid (UA), urea, and pH in urine samples using a single working electrode.
  • Detection ranges: UA (5.0–600 μM), pH (4.0–8.0), urea (0.5–7.0 mM).
  • Detection limits: UA (0.05 μM), urea (5.4 μM); pH sensitivity: 73 mV/pH.
  • Effective mitigation of pH interference through calibration.

Conclusions:

  • The developed tip-like electrochemical sensor offers a simple fabrication method and semi-automatic operation for POCT.
  • The novel multi-component sensing strategy enables simultaneous detection of multiple analytes (UA, urea, pH) with high sensitivity and accuracy.
  • This approach addresses limitations of existing electrochemical sensors, potentially expanding their application in clinical diagnostics and POCT.