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Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing

Fatemeh Zangane Matin1, Seyedeh Elham Rezatofighi2, Mohammad Roayaei Ardakani1

  • 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, 6135743135, Ahvaz, Iran.

Annals of Clinical Microbiology and Antimicrobials
|August 4, 2021
PubMed
Abstract

Insights

Carbapenem-resistant Uropathogenic Escherichia coli (UPEC) producing metallo-beta-lactamases (MBLs) are a growing global health threat. These resistant strains, carrying genes like blaNDM and blaOXA-48, can readily transfer resistance via plasmids.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Uropathogenic Escherichia coli (UPEC) causes frequent urinary tract infections (UTIs).
  • Antimicrobial resistance (AMR), particularly carbapenem resistance (CR), poses a significant global health challenge.
  • Metallo-beta-lactamases (MBLs) are key enzymes conferring resistance to carbapenems.

Purpose of the Study:

  • To investigate the characteristics of MBL-producing UPEC isolates.
  • To identify the prevalence of MBL genes and virulence factors in CR UPEC.
  • To assess the transferability of carbapenem resistance among UPEC strains.

Main Methods:

  • Cross-sectional study of UPEC isolates from October 2018 to December 2019 in Ahvaz, Iran.
  • Detection of MBL production using modified carbapenem inactivation (mCIM) and EDTA-CIM (eCIM) tests.
  • Molecular identification of MBL, phylogenetic, and virulence genes; conjugation assays; plasmid profiling; and ERIC-PCR for clonal analysis.

Main Results:

  • 12 (2.95%) of 406 UPEC isolates were carbapenem-resistant, with 11 being MBL-producing.
  • Prevalent MBL genes included blaNDM (9 isolates) and blaOXA-48 (5 isolates).
  • Key virulence genes identified were traT, fyuA, fimH, and iutA; CR was transferable via plasmids.

Conclusions:

  • The rapid spread of MBL and carbapenemase genes (e.g., blaNDM, blaOXA-48) in UPEC presents a global public health threat.
  • Continuous monitoring of emerging AMR is crucial for updating antimicrobial therapy guidelines.
  • Understanding resistance and virulence mechanisms is vital for developing novel therapeutic strategies against CR UPEC.
Keywords:
Antimicrobial resistanceBla NDMBla OXA-48Carbapenem resistanceCarbapenemaseMetallo-beta-lactamaseUrinary tract infectionUropathogenic Escherichia coliVirulence

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