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The Sampling and Analysis of Human Livers
1National Bureau of Standards, Gaithersburg, MD 20899.
Summary
A pilot program developed a comprehensive method for analyzing human livers for the National Environmental Specimen Bank (NESB). This involved advanced techniques to ensure accurate trace element distribution data.
Area of Science:
- Environmental Health
- Analytical Chemistry
- Toxicology
Background:
- Established in 1980, the pilot National Environmental Specimen Bank (NESB) program focuses on human liver analysis.
- The program addresses the need for standardized protocols in biological specimen banking.
Purpose of the Study:
- To develop and implement a comprehensive approach for the analysis of human livers within the NESB pilot program.
- To establish robust protocols for collection, processing, storage, and analysis ensuring data validity.
Main Methods:
- Utilized cleanroom technology, specialized implements, and inert materials (titanium, Teflon) for sample handling.
- Employed flash-freezing and liquid nitrogen for specimen preservation.
- Implemented a multi-technique analytical scheme, prominently featuring neutron activation analysis.
Main Results:
- Successfully analyzed 66 human livers.
- Determined the distribution of 29 trace elements.
- Validated analytical methods ensuring reliable results for environmental monitoring.
Conclusions:
- A validated, comprehensive methodology for human liver analysis in environmental specimen banking has been established.
- The developed protocols ensure the integrity and accuracy of trace element data for long-term environmental health studies.
