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Testing for Prohibited Substances in Rural Agricultural Show Exhibits: A Twenty-Three Year Experience
Mark Schembri1, Craig Suann2, John Keledjian3
1Department of Obstetrics and Gynaecology, Royal North Shore Hospital, Reserve Road, St Leonards, NSW 2065, Australia.
Journal of Analytical Toxicology
|August 4, 2021
Summary
Drug testing in animal competitions revealed prohibited substance use in 4.7% of animals. While random screening detected some violations, targeted testing showed higher positive rates, indicating a need for ongoing vigilance.
Area of Science:
- Veterinary pharmacology
- Animal welfare science
- Sports integrity
Background:
- Prohibited substance administration in animal competitions undermines fair play.
- Detection of illicit drug use is crucial for maintaining ethical standards in animal sports.
Purpose of the Study:
- To prospectively investigate drug testing outcomes in four animal species over 23 years.
- To identify commonly detected prohibited substances and compare testing strategies.
Main Methods:
- A large-scale, prospective descriptive study involving 1,598 animals across four species.
- Analysis of drug testing results from both random screening and targeted testing protocols.
Main Results:
- Overall, 4.7% of tested animals returned positive results for prohibited substances.
- Commonly detected drugs included acepromazine and phenylbutazone, often legitimate veterinary therapeutics.
- Targeted testing yielded a significantly higher positive rate (50%) compared to random screening (4.7%), though sample sizes differed.
Conclusions:
- Random drug testing effectively identifies a portion of animals using prohibited substances.
- The prevalence of both legitimate therapeutics and other drugs necessitates continuous adaptation of detection methods.
- Ongoing vigilance and research are essential to combat evolving drug use in animal competitions.

