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Challenging the 30-min rule for thawed plasma.

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Short temperature excursions do not compromise thawed plasma quality. Storing thawed plasma (FP) for up to 5 days is safe even with brief temperature fluctuations, ensuring transfusion readiness.

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Area of Science:

  • Transfusion Medicine
  • Blood Product Safety
  • Microbiology

Background:

  • Frozen plasma (FP) requires thawing before transfusion and is stored refrigerated for up to 5 days.
  • Understanding the impact of temperature excursions on thawed plasma quality and safety is crucial for clinical practice.

Purpose of the Study:

  • To evaluate the effect of short-term temperature excursions on the quality and bacterial safety of thawed plasma during its 5-day refrigerated storage.

Main Methods:

  • Thawed plasma units were subjected to controlled temperature excursions (30 or 60 minutes at room temperature) on storage days 0 and 2.
  • Coagulation factor stability (FV, FVII, FVIII, fibrinogen, PT) and bacterial growth (Serratia liquefaciens, Pseudomonas species, Staphylococcus epidermidis) were assessed on day 5.
  • Control units were maintained under refrigerated or room temperature conditions throughout storage.

Main Results:

  • No significant differences in coagulation factor stability were observed between plasma units exposed to 30 or 60-minute temperature excursions and controls.
  • Serratia liquefaciens proliferated in cold-stored plasma, while other tested bacteria survived but did not grow.
  • Bacterial concentrations did not statistically differ between the T30 and T60 groups.

Conclusions:

  • Limited exposure of thawed plasma to uncontrolled room temperatures for up to 60 minutes does not impact coagulation factor stability.
  • Short-term temperature excursions do not promote significant bacterial growth in thawed plasma stored for 5 days.
  • Current storage guidelines for thawed plasma appear adequate to maintain quality and safety despite brief temperature fluctuations.