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Updated: Oct 25, 2025

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method
Published on: March 31, 2022
Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching
Gang Xiang1, Hans Leemhuis1,2, Marc Jos Elise Cornelis van der Maarel1
1Bioproduct Engineering, Engineering and Technology institute Groningen (ENTEG), University of Groningen, Groningen, the Netherlands.
Abstract:
Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.
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