EGR1 promoted anticancer effects of Scutellarin via regulating LINC00857/miR-150-5p/c-Myc in osteosarcoma

Related Experiment Video

Updated: Oct 25, 2025

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.6K

Jian Han1, Peng Wang1, Xin Xia1

  • 1The Second Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China.

Journal of Cellular and Molecular Medicine
|August 4, 2021
PubMed

Insights

Scutellarin, a compound from Erigeron breviscapus, inhibits osteosarcoma growth by boosting EGR1. This pathway downregulates LINC00857, impacting miR-150-5p and c-Myc, offering potential for osteosarcoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Scutellarin, a flavone from Erigeron breviscapus, shows anticancer properties.
  • The specific mechanisms of Scutellarin in osteosarcoma are not well understood.

Purpose of the Study:

  • To investigate the effects and underlying molecular mechanisms of Scutellarin on osteosarcoma.
  • To explore the potential of Scutellarin as a therapeutic agent for osteosarcoma.

Main Methods:

  • Cell growth assays, apoptosis analysis, and tumorigenesis inhibition studies were performed.
  • Expression levels of EGR1, LINC00857, miR-150-5p, and c-Myc were analyzed.
  • Mechanistic studies explored the regulatory axis involving these molecules.

Main Results:

  • Scutellarin suppressed osteosarcoma cell proliferation, induced apoptosis, and inhibited tumor formation.
  • Scutellarin treatment upregulated EGR1, which transcriptionally downregulated LINC00857.
  • LINC00857 was found to sponge miR-150-5p, leading to increased c-Myc; Scutellarin reduced c-Myc by decreasing LINC00857.

Conclusions:

  • The EGR1/LINC00857/miR-150-5p/c-Myc axis is crucial for Scutellarin's anticancer effects in osteosarcoma.
  • Scutellarin demonstrates potential for clinical application in treating osteosarcoma.
Keywords:
EGR1LncRNAosteosarcomascutellarin

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.3K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.6K

Related Concept Videos

The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.4K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.0K