The COVIDome Explorer researcher portal

Area of Science:

• Immunology
• Genomics
• Metabolomics
• Proteomics
• Systems Biology

Background:

• COVID-19 pathology involves complex molecular and cellular dysregulation.
• Integrated research is crucial for understanding and combating the disease.
• Existing datasets often lack multi-omics integration and accessible analysis tools.

Purpose of the Study:

• To create a comprehensive, multidimensional dataset (COVIDome) of hospitalized COVID-19 patients.
• To develop an interactive online portal (COVIDome Explorer) for real-time data analysis and visualization.
• To demonstrate the utility of the COVIDome through multi-omics analysis of C-reactive protein (CRP) biosignatures.

Main Methods:

• Collected multi-omics data: whole-blood transcriptome, plasma proteomics, cytokine profiling, metabolomics (plasma and red blood cells), and immune cell phenotyping (mass cytometry).
• Integrated omics data with clinical annotations.
• Developed the COVIDome Explorer, a web-based platform for interactive data exploration.
• Performed multi-omics analysis to identify biosignatures associated with C-reactive protein (CRP) levels.

Main Results:

• The COVIDome dataset integrates diverse molecular, cellular, and clinical data from COVID-19 patients.
• The COVIDome Explorer provides a user-friendly interface for real-time analysis and visualization of complex COVID-19 data.
• Analysis revealed significant associations between CRP levels and damage-associated molecular patterns, serpin depletion, and mitochondrial metabolism alterations.

Conclusions:

• The COVIDome and its associated Explorer platform offer a powerful resource for COVID-19 research.
• This integrated approach facilitates rapid data sharing, hypothesis testing, and discovery.
• The findings highlight specific molecular pathways linked to disease severity, as exemplified by CRP-associated changes.