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Olfactory Assays for Mouse Models of Neurodegenerative Disease
Published on: August 25, 2014
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Olfactory dysfunction in LATY136F knock-in mice
Misako Kaneda1, Sayaka Yagi-Nakanishi1, Fumi Ozaki1
1Division of Otolaryngology, Head and Neck Surgery, Kanazawa University Graduate School of Medical Sciences, 13-1, Takara-Machi, Kanazawa, Ishikawa 920-8641, Japan.
Auris, Nasus, Larynx
|August 5, 2021
Summary
LATY136F knock-in mice exhibit olfactory dysfunction, characterized by reduced olfactory epithelium regeneration. This study investigated the underlying pathogenic mechanisms in these mice.
Area of Science:
- Neuroscience
- Genetics
- Olfactory Research
Background:
- Olfactory dysfunction is a common symptom in various neurological conditions.
- Understanding the genetic basis of olfactory deficits is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate olfactory dysfunction in LATY136F knock-in mice.
- To elucidate the pathogenic mechanisms contributing to olfactory disturbances in this model.
Main Methods:
- Behavioral tests using cycloheximide solution to assess olfactory function in LATY136F knock-in and wild-type (WT) mice across different ages.
- Immunohistochemical analysis of olfactory epithelium following behavioral assessments.
Main Results:
- LATY136F knock-in mice displayed statistically significant olfactory dysfunction compared to WT mice (P < 0.05).
- Histological analysis revealed thinner olfactory epithelium in knock-in mice.
- Reduced expression of olfactory marker protein and growth-associated protein 43 was observed in knock-in mice (P < 0.05).
Conclusions:
- Olfactory disturbances are confirmed in LATY136F knock-in mice.
- The pathogenic mechanism is suggested to involve impaired regeneration of the olfactory epithelium.
